Assessment of attribution algorithms for resolving CIN3-related HPV genotype prevalence in mixed-genotype biopsy specimens using laser capture microdissection as the reference standard
Alyssa M Cornall, Julia ML Brotherton, Emma T Callegari, Fiona H Tan, Marion Saville, Jan Pyman, Samuel Phillips, Michael J Malloy, Sepehr N Tabrizi, Suzanne M Garland
VACCINE | ELSEVIER SCI LTD | Published : 2020
To make accurate determinations regarding potential and actual impact of HPV vaccine programs, precise estimates of genotype-specific contributions to disease are required for pre- and post-vaccine populations. Definitive determination of lesion-specific genotypes, particularly where multiple genotypes are detected in a sample, can be technically demanding and resource intensive; therefore, most prevalence studies use mathematical algorithms to adjust for multiple genotype detections. There are currently several algorithms, which can produce genotype estimates within a wide range of variability. The use of these for cervical cytology samples has recently been assessed for accuracy against a ..View full abstract
This work was supported with funding from the Victorian Cancer Agency (VCA) (Australia). The funding source had no role in study design, or in the collection, analysis or interpretation of data.