Journal article

Assessment of attribution algorithms for resolving CIN3-related HPV genotype prevalence in mixed-genotype biopsy specimens using laser capture microdissection as the reference standard

Alyssa M Cornall, Julia ML Brotherton, Emma T Callegari, Fiona H Tan, Marion Saville, Jan Pyman, Samuel Phillips, Michael J Malloy, Sepehr N Tabrizi, Suzanne M Garland

VACCINE | ELSEVIER SCI LTD | Published : 2020

Abstract

To make accurate determinations regarding potential and actual impact of HPV vaccine programs, precise estimates of genotype-specific contributions to disease are required for pre- and post-vaccine populations. Definitive determination of lesion-specific genotypes, particularly where multiple genotypes are detected in a sample, can be technically demanding and resource intensive; therefore, most prevalence studies use mathematical algorithms to adjust for multiple genotype detections. There are currently several algorithms, which can produce genotype estimates within a wide range of variability. The use of these for cervical cytology samples has recently been assessed for accuracy against a ..

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