HPV16/18 prevalence in high-grade cervical lesions in an Australian population offered catch-up HPV vaccination
Alyssa M Cornall, Marion Saville, Jan Pyman, Emma T Callegari, Fiona H Tan, Julia Ml Brotherton, Michael J Malloy, Sepehr N Tabrizi, C David Wrede, Suzanne M Garland
VACCINE | ELSEVIER SCI LTD | Published : 2020
OBJECTIVES: Using laser capture microdissection (LCM) and sensitive human papillomavirus (HPV) genotyping, we aimed to determine the distribution of vaccine-preventable types in cervical intraepithelial neoplasia grade 3 (CIN3) lesions and adenocarcinoma in situ (AIS) in young women in Victoria, Australia, offered catch-up HPV vaccination, as a baseline for ongoing vaccine impact monitoring. We also compared findings with available pre-vaccination estimates from women with HPV detected on concurrently-collected cytology samples. METHODS: Consecutive histologically-confirmed CIN3/AIS biopsies were collected between May 2011 and December 2014 from vaccine-eligible women (born after 30th June 1..View full abstract
This work was supported with funding from the Victorian Cancer Agency (VCA). The funding source had no role in study design, or in the collection, analysis or interpretation of data. We thank the Anatomical Pathology Department at Royal Melbourne Hospital for providing slide-imaging services and Elizabeth McKinnon (Department of Anatomical Pathology, Royal Children's Hospital, Melbourne) and Eileen Tan (VCS) who sectioned the tissue blocks and prepared the slides. We thank Tania Tabone for assistance in sub study B set up, Elisa Young, Sarah Osborne and David Hawkes for project co-ordination and Houda Abdo, Angela Hurley, Fiona Tan, Sam Phillips and Marin Poljak for sample preparation and genotype analysis, and Dorothy Machalek for advice on statistical analysis. We acknowledge the initial assistance and advice of LCM methods from Wim H. Quint.