Journal article

Proteomic profiling of developing wheat heads under water-stress

Shahidul Islam, Penghao Wang, Delphine Vincent, Javed M Khan, Angela Juhasz, Dean Diepeveen, Richard J Lipscombe, Wujun Ma, Rudi Appels

FUNCTIONAL & INTEGRATIVE GENOMICS | SPRINGER HEIDELBERG | Published : 2020

Abstract

A replicated iTRAQ (isobaric tags for relative and absolute quantification) study on developing wheat heads from two doubled haploid (DH) lines identified from a cross between cv Westonia x cv Kauz characterized the proteome changes influenced by reproductive stage water-stress. All lines were exposed to 10 days of water-stress from early booting (Zadok 40), with sample sets taken from five head developmental stages. Two sample groups (water-stressed and control) account for 120 samples that required 18 eight-plex iTRAQ runs. Based on the IWGSC RefSeq v1 wheat assembly, among the 4592 identified proteins, a total of 132 proteins showed a significant response to water-stress, including the do..

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University of Melbourne Researchers

Grants

Awarded by Australia China Centre forWheat Improvement (ACCWI), Murdoch University, through the Australian Government, Department of Industry, Innovation, Climate Change, Science, Research, and Tertiary Education, Australia China Science and Research Fund Group Missi


Funding Acknowledgements

This work was supported by the Australia China Centre forWheat Improvement (ACCWI), Murdoch University, through the Australian Government, Department of Industry, Innovation, Climate Change, Science, Research, and Tertiary Education, Australia China Science and Research Fund Group Mission (Funding Agreement ACSRF00542).