Immune profiling of influenza-specific B- and T-cell responses in macaques using flow cytometry-based assays

Marios Koutsakos; Toshiki Sekiya; Brendon Y Chua; Hoang Oanh Nguyen Thi; Adam K Wheatley; Jennifer A Juno; Marumi Ohno; Naoki Nomura; Yuki Ohara; Tomohiro Nishimura; Masafumi Endo; Saori Suzuki; Hirohito Ishigaki; Misako Nakayama; Cong T Nguyen; Yasushi Itoh; Masashi Shingai; Kazumasa Ogasawara; Yoichiro Kino; Stephen J Kent; David C Jackson; Lorena E Brown; Hiroshi Kida; Katherine Kedzierska

Journal article

Immune profiling of influenza-specific B- and T-cell responses in macaques using flow cytometry-based assays

Marios Koutsakos, Toshiki Sekiya, Brendon Y Chua, Hoang Oanh Nguyen Thi, Adam K Wheatley, Jennifer A Juno, Marumi Ohno, Naoki Nomura, Yuki Ohara, Tomohiro Nishimura, Masafumi Endo, Saori Suzuki, Hirohito Ishigaki, Misako Nakayama, Cong T Nguyen, Yasushi Itoh, Masashi Shingai, Kazumasa Ogasawara, Yoichiro Kino, Stephen J Kent Show all

Immunology & Cell Biology | WILEY | Published : 2020

DOI: 10.1111/imcb.12383

Abstract

Influenza remains a significant global public health burden, despite substantial annual vaccination efforts against circulating virus strains. As a result, novel vaccine approaches are needed to generate long-lasting and universal broadly cross-reactive immunity against distinct influenza virus strains and subtypes. Several new vaccine candidates are currently under development and/or in clinical trials. The successful development of new vaccines requires testing in animal models, other than mice, which capture the complexity of the human immune system. Importantly, following vaccination or challenge, the assessment of adaptive immunity at the antigen-specific level is particularly informati..

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University of Melbourne Researchers

Jennifer Juno Author Microbiology and Immunology

Lorena Brown Author Microbiology and Immunology

Adam Wheatley Author Microbiology and Immunology

Stephen Kent Author Microbiology and Immunology

Toshiki Sekiya Author Microbiology and Immunology

Grants

Awarded by Japan Agency for Medical Research and Development (AMED)

Awarded by Australian National Health and Medical Research Council (NHMRC) Program

Awarded by NHMRC Senior Research Fellowship Level B

Awarded by NHMRC Investigator Grant

Funding Acknowledgements

We thank Dr Watanabe (National Institute of Infectious Disease in Japan) for kindly providing influenza virus strains, A/Singapore/GP1908/2015 (IVR-180) (H1N1) pdm09 and A/Narita/1/2009 (H1N1), and also Dr Kawakami (Yokohama City Institute of Public Health) for A/Yokohama/91/2007 (H1N1). We also thank Ms Kitagawa, Ms Sasamura and Mr Ishida (Shiga University of Medical Science) for technical assistance. The project was supported by the Japan Agency for Medical Research and Development (AMED) under grant numbers JP19fm0108008 (Japan Initiative for Global Research Network on Infectious Diseases) and JP20wm0125008 (Japan Program for Infectious Diseases Research and Infrastructure), and by the GI-CoRE Program of Hokkaido University. The work was funded by the Australian National Health and Medical Research Council (NHMRC) Program Grant (1071916) to KK, DCJ and LEB. KK was supported by an NHMRC Senior Research Fellowship Level B (Grant No. 1102792), NHMRC Investigator Grant (Grant No. 1173871) and the University of Melbourne Dame Kate Campbell Fellowship.