Journal article

Template-Mediated Assembly of DNA into Microcapsules for Immunological Modulation

Yijiao Qu, Yi Ju, Christina Cortez-Jugo, Zhixing Lin, Shiyao Li, Jiajing Zhou, Yutian Ma, Agata Glab, Stephen J Kent, Francesca Cavalieri, Frank Caruso

SMALL | WILEY-V C H VERLAG GMBH | Published : 2020

Abstract

There is a need for effective vaccine delivery systems and vaccine adjuvants without extraneous excipients that can compromise or minimize their efficacy. Vaccine adjuvants cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODNs) can effectively activate immune responses to secrete cytokines. However, CpG ODNs are not stable in serum due to enzymatic cleavage and are difficult to transport through cell membranes. Herein, DNA microcapsules made of CpG ODNs arranged into 3D nanostructures are developed to improve the serum stability and immunostimulatory effect of CpG. The DNA microcapsules allow encapsulation and co-delivery of cargoes, including glycogen. The DNA capsules, with >4 milli..

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Grants

Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology


Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by NHMRC Senior Principal Research Fellowships


Funding Acknowledgements

This research was conducted and funded by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036) and the Australian National Health and Medical Research Council (NHMRC; Program Grant GNT1149990). F. Caruso and S.J.K. acknowledge the award of NHMRC Senior Principal Research Fellowships (GNT1135806 and GNT1136322, respectively). Y.Q. acknowledges support from the China Scholarship Council (CSC). F. Cavalieri acknowledges the award of an RMIT Senior Vice Chancellor Fellowship. This work was performed in part at the Materials Characterisation and Fabrication Platform (MCFP) at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF). The authors thank Sukhvir Kaur Bhangu for assistance with experiments, Paul Brannon for assistance with the microscopy experiments, and Dr. Gyeongwon Yun and Dr. Joseph J. Richardson for helpful discussions.