Journal article

Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos

Thomas Shafee, Antony Bacic, Kim Johnson

Molecular Biology and Evolution | OXFORD UNIV PRESS | Published : 2020


Approaches for studying the evolution of globular proteins are now well established yet are unsuitable for disordered sequences. Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships. Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these. We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family,..

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University of Melbourne Researchers


Awarded by La Trobe Research Focus Area

Funding Acknowledgements

T.S., A.B., andK.J. were supported by the La Trobe Institute for Agriculture and Food and K.J. by La Trobe Research Focus Area (Grant No. 2000004372). The authors would like to acknowledge Yingxuan Ma and Monika Doblin who provided constructive advice on the manuscript. A.B. and K.J. would like to acknowledge a startup grant for the Sino-Australia Plant Cell Wall Research Centre provided by Zhejiang A&F University.