Journal article

Effect of benzalkonium chloride addition to EDTA on attachment and proliferation of dental pulp stem cells on dentin and on transforming growth factor-beta 1 release

Selen Kucukkaya Eren, Elham Bahador Zirh, Naciye Dilara Zeybek, Sevinc Askerbeyli Ors, Hacer Aksel, Peter Parashos

Odontology | SPRINGER | Published : 2020

Abstract

To investigate the effect of benzalkonium chloride (BAC) addition to ethylenediaminetetraacetic acid (EDTA) on transforming growth factor-β1 (TGF-β1) release, as well as attachment and proliferation of dental pulp stem cells (DPSCs) on dentin. A total of standard 268 human dentin disks were prepared and immersed in 1.5% sodium hypochlorite (NaOCl) for 5 min. The disks were rinsed with distilled water and randomly divided into seven groups. In control group, the disks received no further treatment. The remaining disks were immersed in following solutions: 17% EDTA or 17% EDTA + 0.008% BAC for 1, 5 or 10 min and rinsed with distilled water. DPSCs were seeded in part of the disks since the TGF-..

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University of Melbourne Researchers

Grants

Awarded by Hacettepe University Scientific Research Projects Coordination Unit


Funding Acknowledgements

This research was supported by the Hacettepe University Scientific Research Projects Coordination Unit (THD-2018-16215).