A Minimally Invasive Experimental Model of Acute Ocular Hypertension with Acute Angle Closure Characteristics.
Rachel S Chong, Joanna MF Busoy, Bingyao Tan, Sia Wey Yeo, Ying Shi Lee, Amutha V Barathi, Jonathan G Crowston, Leopold Schmetterer
Translational Vision Science and Technology | Published : 2020
Purpose: To describe a minimally invasive experimental model of acute ocular hypertension (OHT) with characteristics of acute angle closure (AAC). Methods: Adult C57/Bl6 mice (n = 31) were subjected to OHT in one eye using a modified circumlimbal suture technique that elevated intraocular pressure (IOP) for 30 minutes. Contralateral un-operated eyes served as controls. IOP, anterior segment optical coherence tomography, and fundus fluorescein angiography (FFA) were performed. The positive scotopic threshold response (pSTR) and a-wave and b-wave amplitudes were also evaluated. Retinal tissues were immunostained for the retinal ganglion cell (RGC) marker RBPMS and the glial marker GFAP. Result..View full abstract