Collagen VI alpha 2 chain deficiency causes trabecular bone loss by potentially promoting osteoclast differentiation through enhanced TNF alpha signaling
Hai T Pham, Vardit Kram, Qurratul-Ain Dar, Taishi Komori, Youngmi Ji, Payam Mohassel, Jachinta Rooney, Li Li, Tina M Kilts, Carsten Bonnemann, Shireen Lamande, Marian F Young
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2020
Type VI collagen is well known for its role in muscular disorders, however its function in bone is still not well understood. To examine its role in bone we analyzed femoral and vertebral bone mass by micro-computed tomography analysis, which showed lower bone volume/total volume and trabecular number in Col6α2-KO mice compared with WT. Dynamic histomorphometry showed no differences in trabecular bone formation between WT and Col6α2-KO mice based on the mineral appositional rate, bone formation rate, and mineralizing perimeter. Femoral sections were assessed for the abundance of Tartrate Resistant Acid Phosphatase-positive osteoclasts, which revealed that mutant mice had more osteoclasts com..View full abstract
Awarded by NIH, NIDCR
The research was supported in part by the Intramural Research Program of the NIH, NIDCR Molecular Biology of Bones and Teeth Section (Z01DE000379-35), Veterinary Resources Core (ZICDE000740-05) Genomics and Computational Biology Core (ZIC DC000086), Imaging Core (ZIC DE000750-01) Combined Technical Research Core (ZIC DE000729-09).