Analysis of Hepatitis B Virus Haplotype Diversity Detects Striking Sequence Conservation Across Genotypes and Chronic Disease Phase

J Wagner; L Yuen; M Littlejohn; V Sozzi; K Jackson; V Suri; S Tan; B Feierbach; A Gaggar; P Marcellin; M Buti Ferret; HLA Janssen; E Gane; HLY Chan; D Colledge; G Rosenberg; J Bayliss; BP Howden; SA Locarnini; D Wong; AT Thompson; PA Revill

Journal article

Analysis of Hepatitis B Virus Haplotype Diversity Detects Striking Sequence Conservation Across Genotypes and Chronic Disease Phase

J Wagner, L Yuen, M Littlejohn, V Sozzi, K Jackson, V Suri, S Tan, B Feierbach, A Gaggar, P Marcellin, M Buti Ferret, HLA Janssen, E Gane, HLY Chan, D Colledge, G Rosenberg, J Bayliss, BP Howden, SA Locarnini, D Wong Show all

Hepatology | WILEY | Published : 2021

DOI: 10.1002/hep.31516

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Abstract

Background and Aims: We conducted haplotype analysis of complete hepatitis B virus (HBV) genomes following deep sequencing from 368 patients across multiple phases of chronic hepatitis B (CHB) infection from four major genotypes (A-D), analyzing 4,110 haplotypes to identify viral variants associated with treatment outcome and disease progression. Approach and Results: Between 18.2% and 41.8% of nucleotides and between 5.9% and 34.3% of amino acids were 100% conserved in all genotypes and phases examined, depending on the region analyzed. Hepatitis B e antigen (HBeAg) loss by week 192 was associated with different haplotype populations at baseline. Haplotype populations differed across the HB..

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