Journal article

Microbe-Metabolite Associations Linked to the Rebounding Murine Gut Microbiome Postcolonization with Vancomycin-Resistant Enterococcus faecium

Andre Mu, Glen P Carter, Lucy Li, Nicole S Isles, Alison F Vrbanac, James T Morton, Alan K Jarmusch, David P De Souza, Vinod K Narayana, Komal Kanojia, Brunda Nijagal, Malcolm J McConville, Rob Knight, Benjamin P Howden, Timothy P Stinear

MSYSTEMS | AMER SOC MICROBIOLOGY | Published : 2020

Abstract

Vancomycin-resistant Enterococcus faecium (VREfm) is an emerging antibiotic-resistant pathogen. Strain-level investigations are beginning to reveal the molecular mechanisms used by VREfm to colonize regions of the human bowel. However, the role of commensal bacteria during VREfm colonization, in particular following antibiotic treatment, remains largely unknown. We employed amplicon 16S rRNA gene sequencing and metabolomics in a murine model system to try and investigate functional roles of the gut microbiome during VREfm colonization. First-order taxonomic shifts between Bacteroidetes and Tenericutes within the gut microbial community composition were detected both in response to pretreatme..

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Grants

Awarded by Endeavor Research Fellowship (Australia Awards) for collaborative research in the Department of Pediatrics at UCSD, CA, USA


Awarded by National Medical Research Council of Australia


Funding Acknowledgements

The project was supported by an Endeavor Research Fellowship (Australia Awards) for collaborative research in the Department of Pediatrics at UCSD, CA, USA (ERF PDR 6735_2018) (A.M.). The project was also supported by the National Medical Research Council of Australia, GNT1105525 (T.P.S.) and GNT 1026656 (B.P.H.).