Journal article
Combined blockade of ADP receptors and PI3-kinase p110β fully prevents platelet and leukocyte activation during hypothermic extracorporeal circulation
S Krajewski, J Kurz, T Geisler, K Peter, HP Wendel, A Straub
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012
Abstract
Extracorporeal circulation (ECC) and hypothermia are used to maintain stable circulatory parameters and improve the ischemia tolerance of patients in cardiac surgery. However, ECC and hypothermia induce activation mechanisms in platelets and leukocytes, which are mediated by the platelet agonist ADP and the phosphoinositide-3-kinase (PI3K) p110β. Under clinical conditions these processes are associated with life-threatening complications including thromboembolism and inflammation. This study analyzes effects of ADP receptor P2Y12 and P2Y1 blockade and PI3K p110β inhibition on platelets and granulocytes during hypothermic ECC. Human blood was treated with the P2Y12 antagonist 2-MeSAMP, the P2..
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Awarded by German Research Association (Deutsche Forschungsgemeinschaft)
Funding Acknowledgements
This study was funded by the German Research Association (Deutsche Forschungsgemeinschaft; Grant number: STR 687/2-1). K. Peter was supported by a Future Fellowship of the Australian Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.