Journal article
Imaging monocytes with iron oxide nanoparticles targeted towards the monocyte integrin MAC-1 (CD11b/CD18) does not result in improved atherosclerotic plaque detection by in vivo MRI
C Von Zur Muhlen, A Fink-Petri, J Salaklang, D Paul, I Neudorfer, V Berti, A Merkle, K Peter, C Bode, D Von Elverfeldt
Contrast Media and Molecular Imaging | JOHN WILEY & SONS LTD | Published : 2010
DOI: 10.1002/cmmi.384
Abstract
Imaging of macrophages with superparamagnetic iron oxide particles (SPIO) has been performed to improve detection of atherosclerotic plaque inflammation in human and mouse studies by molecular magnetic resonance imaging (MRI). Since affinity of the monocyte/macrophage integrin MAC-1 (CD11b/CD18) is upregulated in inflammation, we generated a contrast agent targeting CD11b (CD11b-SPIOs) for improved macrophage detection in plaques. CD11b-SPIOs and non-targeted SPIOs (control-SPIOs) were incubated in vitro with human monocytes/macrophages. As quantified by SPIO-induced MRI signal extinction, intracellular iron-content was significantly higher in monoytes/macrophages incubated with CD11b-SPIO t..
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Awarded by German Research Foundation (DFG)
Awarded by BMBF
Funding Acknowledgements
This work was funded by a grant of the German Research Foundation (DFG, grant no. MU 2727/1-3) and the BMBF (grant no. 13N8873).