Improving selection of αIIbβ3-binding phage antibodies with increased reactivity derived from immunized donors

Abstract

Although many studies of the immune response in polytransfused Glanzmann thrombasthenia (GT) patients and in autoimmune thrombocytopenic purpura (AITP) have demonstrated the frequent development of Abs directed against the αIIbβ3 integrin, little is known about the induced anti-αIIbβ3 autoantibodies at the molecular level. Phage display is a powerful technology for selecting and engineering mAbs expressed on the surface of filamentous bacteriophage. Combinatorial libraries of single-chain IgG were constructed from splenocytes from two patients with AITP and one patient with GT. In a previous study, activated platelets or αIIbβ3-expressing CHO cells selection was performed to isolate human Ig..