Journal article
Visualization of activated platelets by targeted magnetic resonance imaging utilizing conformation-specific antibodies against glycoprotein IIb/IIIa
C Von Zur Muhlen, K Peter, ZA Ali, JE Schneider, MA McAteer, S Neubauer, KM Channon, C Bode, RP Choudhury
Journal of Vascular Research | KARGER | Published : 2008
DOI: 10.1159/000135660
Abstract
Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to image platelets. MPIO (size: 1 μm) were conjugated to anti-LIBS or control single-chain antibody. Following guidewire injury to mouse femoral artery, platelet adhesion was present after 24 h. Mice were perfused with anti-LIBS-MPIO (or control MPIO) via the left ventricle and 11.7-tesla MRI was performed on femoral arteries ex vivo. A 3D gradient echo sequence attained an isotrop..
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Funding Acknowledgements
The authors gratefully acknowledge the contribution of Mr. Phil Townsend, Ms. Julia Moeller and Ms. Irene Neudorfer. We thank Dr. Susan Smyth (Chapel Hill, N.C., USA) for donating the anti-P-selectin antibody. Prof. Kieran Clarke is thanked for enabling the MRI studies.The study was supported by the Wellcome Trust (London, UK) and the National Heart Foundation (Australia).