Journal article
Simultaneous structural and elemental nano-imaging of human brain tissue
S Genoud, MWM Jones, BG Trist, J Deng, S Chen, DJ Hare, KL Double
Chemical Science | ROYAL SOC CHEMISTRY | Published : 2020
DOI: 10.1039/d0sc02844d
Abstract
Examining chemical and structural characteristics of micro-features in complex tissue matrices is essential for understanding biological systems. Advances in multimodal chemical and structural imaging using synchrotron radiation have overcome many issues in correlative imaging, enabling the characterization of distinct microfeatures at nanoscale resolution in ex vivo tissues. We present a nanoscale imaging method that pairs X-ray ptychography and X-ray fluorescence microscopy (XFM) to simultaneously examine structural features and quantify elemental content of microfeatures in complex ex vivo tissues. We examined the neuropathological microfeatures Lewy bodies, aggregations of superoxide dis..
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Grants
Awarded by Australian Government
Funding Acknowledgements
We would like to thank the Sydney Microscopy and Microanalysis Facility for their valuable advice and assistance with image analysis. We would also like to thank the London Neurodegenerative Diseases Brain Bank at King's College London and the Multiple Sclerosis and Parkinson's Tissue Bank at Imperial College London for providing access to human tissue samples, as well as the donors and their families. Silicon nitride windows were manufactured at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australia's researchers. The Australian Research Council (ARC) Centre of Excellence for Advanced Molecular Imaging (CE140100011), and National Health and Medical Research Council (NHMRC) grants (GNT1122981; GNT1181864) schemes funded this work. This study was supported by ForeFront, a large collaborative research group dedicated to the study of neurodegenerative diseases and funded by the National Health and Medical Research Council of Australia Program Grant (1132524), Dementia Research Team Grant (1095127), NeuroSleep Centre of Research Excellence (1060992), and the ARC Centre of Excellence in Cognition and its Disorders Memory Program (CE10001021). D. J. H. wishes to acknowledge research and material support from Agilent Technologies through the NHMRC Career Development Industry Fellowship program (GNT1122981). This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. Implementation of the Bionanoprobe was supported by NIH ARRA grant SP0007167. The authors would like to thank Evan Maxey for the technical support at the Bionanoprobe beamline. The authors would also like to acknowledge travel funding provided by the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron, part of ANSTO, and funded by the Australian Government; and supporting funds from Parkinson's NSW.