High antibody titres induced by protein subunit vaccines using Mycobacterium ulcerans antigens Hsp18 and MUL _3720 with a TLR-2 agonist fail to protect against Buruli ulcer in mice
Kirstie M Mangas, Nicholas J Tobias, Estelle Marion, Jeremie Babonneau, Laurent Marsollier, Jessica L Porter, Sacha J Pidot, Chinn Yi Wong, David C Jackson, Brendon Y Chua, Timothy P Stinear
PEERJ | PEERJ INC | Published : 2020
Background: Mycobacterium ulcerans is the causative agent of a debilitating skin and soft tissue infection known as Buruli ulcer (BU). There is no vaccine against BU. The purpose of this study was to investigate the vaccine potential of two previously described immunogenic M. ulcerans proteins, MUL_3720 and Hsp18, using a mouse tail infection model of BU. Methods: Recombinant versions of the two proteins were each electrostatically coupled with a previously described lipopeptide adjuvant. Seven C57BL/6 and seven BALB/c mice were vaccinated and boosted with each of the formulations. Vaccinated mice were then challenged with M. ulcerans via subcutaneous tail inoculation. Vaccine performance wa..View full abstract
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Awarded by National Health and Medical Research Council of Australia
This work was supported by the National Health and Medical Research Council of Australia (GNT1105525). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.