Localised Collagen2a1 secretion supports lymphatic endothelial cell migration in the zebrafish embryo
Smrita Chaudhury, Kazuhide S Okuda, Katarzyna Koltowska, Anne K Lagendijk, Scott Paterson, Gregory J Baillie, Cas Simons, Kelly A Smith, Benjamin M Hogan, Neil Bower
Development | COMPANY BIOLOGISTS LTD | Published : 2020
The lymphatic vasculature develops primarily from pre-existing veins. A pool of lymphatic endothelial cells (LECs) first sprouts from cardinal veins followed by migration and proliferation to colonise embryonic tissues. Although much is known about the molecular regulation of LEC fate and sprouting during early lymphangiogenesis, we know far less about the instructive and permissive signals that support LEC migration through the embryo. Using a forward genetic screen, we identified mbtps1 and sec23a, components of the COP-II protein secretory pathway, as essential for developmental lymphangiogenesis. In both mutants, LECs initially depart the cardinal vein but then fail in their ongoing migr..View full abstract
Awarded by Australian Research Council
Awarded by Australian Research Council Future Fellowship
Awarded by National Health and Medical Research Council
This work was supported in part by Australian Research Council discovery grants (DP150103110 and DP180102846). A.K.L. was supported by a University of Queensland Postdoctoral Fellowship. K.K. was supported by a Lymphatic Education & Research Network Postdoctoral Fellowship. K.A.S. was part funded by an Australian Research Council Future Fellowship (FT110100496). B.M.H. was supported by fellowships from the National Heart Foundation and National Health and Medical Research Council (1083811 and 1155221).