Global analysis of adenylate-forming enzymes reveals beta-lactone biosynthesis pathway in pathogenic Nocardia
Serina L Robinson, Barbara R Terlouw, Megan D Smith, Sacha J Pidot, Timothy P Stinear, Marnix H Medema, Lawrence P Wackett
Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2020
Enzymes that cleave ATP to activate carboxylic acids play essential roles in primary and secondary metabolism in all domains of life. Class I adenylate-forming enzymes share a conserved structural fold but act on a wide range of substrates to catalyze reactions involved in bioluminescence, nonribosomal peptide biosynthesis, fatty acid activation, and β-lactone formation. Despite their metabolic importance, the substrates and functions of the vast majority of adenylate-forming enzymes are unknown without tools available to accurately predict them. Given the crucial roles of adenylate-forming enzymes in biosynthesis, this also severely limits our ability to predict natural product structures f..View full abstract
Awarded by National Science Foundation
Awarded by Netherlands Organization for Scientific Research (NWO)
S.L.R. is supported by the National Science Foundation Graduate Research Fellowship under NSF grant number 00039202 and a Graduate Research Opportunities Worldwide (GROW) fellowship to the Netherlands supported by the NSF and the Netherlands Organization for Scientific Research (NWO) grant number 040.15.054/6097 (to S. L. R. and M. H. M.).