The TAZ-CAMTA1 and YAP-TFE3 fusion proteins transform cells by binding to subunits of the histone acetyltransferase Ada2a-containing complex (ATAC).

Abstract

Abstract Background: Epithelioid hemangioendothelioma (EHE) is a vascular sarcoma characterized by WWTR1(TAZ)-CAMTA1 (85%) and YAP1-TFE3 gene fusions (15%). The fusion proteins contain the N-terminus of TAZ and YAP, including their TEAD binding domains, fused in frame to the C-termini of CAMTA1 and TFE3, respectively. The C-termini of CAMTA1 and TFE3 contain transactivating domains as well as other protein-protein interaction domains. The structure of the fusion proteins led us to hypothesize that the C termini of CAMTA1 and TFE3 interact with novel proteins that potentiate the oncogenic activity of TAZ and YAP. Methods: NIH 3T3 and SW872 cell lines were transdu..