Journal article

First record of a tandem-repeat region within the mitochondrial genome ofClonorchis sinensisusing a long-read sequencing approach

Liina Kinkar, Neil D Young, Woon-Mok Sohn, Andreas J Stroehlein, Pasi K Korhonen, Robin B Gasser

PLoS Neglected Tropical Diseases | PUBLIC LIBRARY SCIENCE | Published : 2020

Abstract

BACKGROUND: Mitochondrial genomes provide useful genetic markers for systematic and population genetic studies of parasitic helminths. Although many such genome sequences have been published and deposited in public databases, there is evidence that some of them are incomplete relating to an inability of conventional techniques to reliably sequence non-coding (repetitive) regions. In the present study, we characterise the complete mitochondrial genome-including the long, non-coding region-of the carcinogenic Chinese liver fluke, Clonorchis sinensis, using long-read sequencing. METHODS: The mitochondrial genome was sequenced from total high molecular-weight genomic DNA isolated from a pool of ..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by ARC LIEF Grant


Funding Acknowledgements

Project support was through Australian Research Council (ARC) grants LP180101334 (N. D.Y. and P.K.K.) and LP180101085 (R.B.G.) and Yourgene Health Singapore. N.D.Y. and P.K.K. were recipients of Career Development and Early Career Research Fellowships, respectively, from the National Health and Medical Research Council (NHMRC) of Australia. The LIEF HPC-GPGPU Facility is supported by ARC LIEF Grant LE170100200. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.