Journal article

Long-term single-cell imaging and simulations of microtubules reveal driving forces for wall pattering during proto-xylem development

René Schneider, Kris van ’t Klooster, Kelsey Picard, Jasper van der Gucht, Taku Demura, Marcel Janson, Arun Sampathkumar, Eva Deinum, Tijs Ketelaar, Staffan Persson

Published : 2020


ABSTRACT Plants are the tallest organisms on Earth; a feature sustained by solute-transporting xylem vessels in the plant vasculature. The xylem vessels are supported by strong cell walls that are assembled in intricate patterns. Cortical microtubules direct wall deposition and need to rapidly re-organize during xylem cell development. We established long-term live-cell imaging of single Arabidopsis cells undergoing proto-xylem trans-differentiation, resulting in spiral wall patterns, to investigate the microtubule re-organization. The initial disperse microtubule array rapidly readjusted into well-defined microtubule bands, which required local de-stabilization of individual microtubules in..

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University of Melbourne Researchers