Journal article

A Biobank of Colorectal Cancer Patient-Derived Xenografts

Suad M Abdirahman, Michael Christie, Adele Preaudet, Marie CU Burstroem, Dmitri Mouradov, Belinda Lee, Oliver M Sieber, Tracy L Putoczki

Cancers | MDPI | Published : 2020

Abstract

Colorectal cancer (CRC) is a challenging disease, with a high mortality rate and limited effective treatment options, particularly for late-stage disease. Patient-derived xenografts (PDXs) have emerged as an informative, renewable experimental resource to model CRC architecture and biology. Here, we describe the generation of a biobank of CRC PDXs from stage I to stage IV patients. We demonstrate that PDXs within our biobank recapitulate the histopathological and mutation features of the original patient tumor. In addition, we demonstrate the utility of this resource in pre-clinical chemotherapy and targeted treatment studies, highlighting the translational potential of PDX models in the ide..

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Grants

Awarded by Victorian Cancer Agency


Awarded by National Health and Medical Research Council (NHMRC) of Australia


Funding Acknowledgements

This project was funded by the Victorian Cancer Agency (MCRF16009 to T.L.P.) with additional support from Cancer Therapeutics (CTX to T.L.P. and O.M.S.), and the National Health and Medical Research Council (NHMRC) of Australia (project 1080498 to TLP, project 1079362 and fellowship 1136119 to O.M.S.), a WEHI Dyson Bequest Centenary Fellowship (T.L.P.). We gratefully acknowledge infrastructure support from the NHMRC IRIISS and the Victorian State Government Operational Infrastructure Support Program.