Mechanism and inhibition of the papain-like protease, PLpro, of SARS-CoV-2

Theresa Klemm; Gregor Ebert; Dale J Calleja; Cody C Allison; Lachlan W Richardson; Jonathan P Bernardini; Bernadine GC Lu; Nathan W Kuchel; Christoph Grohmann; Yuri Shibata; Zhong Yan Gan; James P Cooney; Marcel Doerflinger; Amanda E Au; Timothy R Blackmore; Gerbrand J van der Heden van Noort; Paul P Geurink; Huib Ovaa; Janet Newman; Alan Riboldi-Tunnicliffe; Peter E Czabotar; Jeffrey P Mitchell; Rebecca Feltham; Bernhard C Lechtenberg; Kym N Lowes; Grant Dewson; Marc Pellegrini; Guillaume Lessene; David Komander

Journal article

Mechanism and inhibition of the papain-like protease, PLpro, of SARS-CoV-2

Theresa Klemm, Gregor Ebert, Dale J Calleja, Cody C Allison, Lachlan W Richardson, Jonathan P Bernardini, Bernadine GC Lu, Nathan W Kuchel, Christoph Grohmann, Yuri Shibata, Zhong Yan Gan, James P Cooney, Marcel Doerflinger, Amanda E Au, Timothy R Blackmore, Gerbrand J van der Heden van Noort, Paul P Geurink, Huib Ovaa, Janet Newman, Alan Riboldi-Tunnicliffe Show all

The EMBO Journal | WILEY | Published : 2020

DOI: 10.15252/embj.2020106275

University of Melbourne Researchers

Peter Czabotar Author Medical Biology (W.E.H.I.)

Guillaume Lessene Author Medical Biology (W.E.H.I.)

Kym Lowes Author Medical Biology (W.E.H.I.)

Cody Allison Author Medical Biology (W.E.H.I.)

Marcel Doerflinger Author Medical Biology (W.E.H.I.)

Grants

Awarded by Walter and Eliza Hall Institute of Medical Research, an NHMRC/MRFF "VirDUB" grant

Awarded by NHMRC

Awarded by NHMRC Independent Research Institutes Infrastructure Support Scheme grant

Funding Acknowledgements

We would like to thank Kanta Subbarao (Peter Doherty Institute, Melbourne) for live SARS-CoV-2 virus, Frederick P. (Fritz) Roth (University of Toronto) for providing the SARS2 nsp3 pENTRY plasmid, Thibault Major (University of British Columbia, Vancouver) for vectors and for supporting J. Bernardini, Sandra Nicholson, Peter Colman, Ian Wicks and Thomas Cotton (WEHI) for sharing expertise and reagents, and Jonathan O'Connell (FORMA Therapeutics) for advice on high-throughput assays. This work was funded by The Walter and Eliza Hall Institute of Medical Research, an NHMRC/MRFF "VirDUB" grant MRF2002119 (to DK, GL, MP, PEC), NWO (VIDI-grant and Off-road grant) to GJvdHvN, NHMRC Investigator Grants and Fellowships (GNT1178122 to DK, GNT0637350 to MP, and GNT1117089 to GL), NHMRC Independent Research Institutes Infrastructure Support Scheme grant (361646) and Victorian State Government Operational Infrastructure Support grant, and a generous donation by Hengyi Pacific Pty Ltd to support COVID-19 research.