The oligomeric assembly of galectin-11 is critical for anti-parasitic activity in sheep (Ovis aries)
Dhanasekaran Sakthivel, Sarah Preston, Robin B Gasser, Tatiana P Soares da Costa, Julia N Hernandez, Adam Shahine, MD Shakif-Azam, Peter Lock, Jamie Rossjohn, Matthew A Perugini, Jorge Francisco Gonzalez, Els Meeusen, David Piedrafita, Travis Beddoe
Communications Biology | NATURE RESEARCH | Published : 2020
Galectins are a family of glycan-binding molecules with a characteristic affinity for ß-D-glycosides that mediate a variety of important cellular functions, including immune and inflammatory responses. Galectin-11 (LGALS-11) has been recently identified as a mediator induced specifically in animals against gastrointestinal nematodes and can interfere with parasite growth and development. Here, we report that at least two natural genetic variants of LGALS-11 exist in sheep, and demonstrate fundamental differences in anti-parasitic activity, correlated with their ability to dimerise. This study improves our understanding of the role of galectins in the host immune and inflammatory responses ag..View full abstract
We thank staff at the Monash Macromolecular Crystallisation Facility (MMCF) for assistance with crystallisation, and at the Australian Synchrotron for support with X-ray data collection. We are grateful to Dr. Dene Littler for initial support with crystal structure analysis. DS acknowledges support from the Department of State Development, Business and Innovation (DSDBI), Victoria, Australia and Australia-India Institute (AII). DS acknowledges the financial support from the Australian Society for Parasitology to attend CCP4 crystallography workshop. R.B.G.'s research was funded through grants from the Australian Research Council (ARC), the National Health and Medical Research Council (NHMRC) of Australia, Yourgene Bioscience Taiwan, and Melbourne Water Corporation.