Journal article

Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB

Steven J Hancock, Phan Minh-Duy, Zhenyao Luo, Alvin W Lo, Kate M Peters, Thi Khanh Nhu Nguyen, Brian M Forde, Jason Whitfield, Ji Yang, Richard A Strugnell, David L Paterson, Timothy R Walsh, Bostjan Kobe, Scott A Beatson, Mark A Schembri



The IncC family of broad-host-range plasmids enables the spread of antibiotic resistance genes among human enteric pathogens1-3. Although aspects of IncC plasmid conjugation have been well studied4-9, many roles of conjugation genes have been assigned based solely on sequence similarity. We applied hypersaturated transposon mutagenesis and transposon-directed insertion-site sequencing to determine the set of genes required for IncC conjugation. We identified 27 conjugation genes, comprising 19 that were previously identified (including two regulatory genes, acaDC) and eight not previously associated with conjugation. We show that one previously unknown gene, acaB, encodes a transcriptional r..

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Awarded by Australian National Health and Medical Research Council (NHMRC)

Awarded by NHMRC Senior Research Fellowship

Awarded by NHMRC Career Development Fellowship

Awarded by NHMRC Principal Research Fellowship

Awarded by Australian Research Council Laureate Fellowship

Funding Acknowledgements

We acknowledge the scientific and technical assistance of the Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland, the Institute for Molecular Bioscience Sequencing Facility and the Australian Synchrotron MX beamlines, part of ANSTO, and we made use of the Australian Cancer Research Foundation detector. This work was supported by Project (nos. GNT1106590, GNT1033799 and GNT1067455) and Program (no. GNT1071659) grants from the Australian National Health and Medical Research Council (NHMRC). S.J.H. is supported by an Australian Government Research Training Program Scholarship. M.A.S. is supported by an NHMRC Senior Research Fellowship (no. GNT1106930), S.A.B. is supported by a NHMRC Career Development Fellowship (no. GNT1090456) and B.K. is supported by a NHMRC Principal Research Fellowship (no. GNT1110971) and an Australian Research Council Laureate Fellowship (no. FL180100109). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.