Effects of insulin on IGF-1 receptors in equine lamellar tissue in vitro
S Rahnama, R Spence, N Vathsangam, CL Baskerville, SR Bailey, MA de Laat, ST Anderson, CC Pollitt, MN Sillence
Domestic Animal Endocrinology | ELSEVIER SCIENCE INC | Published : 2021
Although it is understood that equine endocrinopathic laminitis can be triggered by high concentrations of insulin, it is unclear whether this represents a direct action on lamellar tissue via insulin receptors (InsR), an interaction with IGF-1 receptors (IGF-1R), or some other, indirect action. This uncertainty is because of the reported scarcity of InsR in lamellar tissue and the low affinity of insulin for equine IGF-1R. In the present study, the effects of insulin and IGF-1 (as a positive control) were examined using lamellar explants isolated from the hooves of healthy horses and incubated in cell culture medium for between 2 min and 48 h. In this system, a low physiological concentrati..View full abstract
Awarded by Australian Research Council Industry Linkage Grant
The authors thank Meramist Abattoir for providing tissue samples and Drs Gunther and Simpson for providing human cell lysates. This study was funded by an Australian Research Council Industry Linkage Grant (LP15101025) in association with Zoetis and the Animal Health Foundation. None of the funding bodies had a role in the study design. The authors declare no conflicts of interest.