Evaluating environmental DNA as a tool for detecting an amphibian pathogen using an optimized extraction method
Laura A Brannelly, Daniel P Wetzel, Michel EB Ohmer, Lydia Zimmerman, Veronica Saenz, Corinne L Richards-Zawacki
OECOLOGIA | SPRINGER | Published : 2020
Environmental DNA (eDNA) detection is a valuable conservation tool that can be used to identify and monitor imperiled or invasive species and wildlife pathogens. Batrachochytrium pathogens are of global conservation concern because they are a leading cause of amphibian decline. While eDNA techniques have been used to detect Batrachochytrium DNA in the environment, a systematic comparison of extraction methods across environmental samples is lacking. In this study, we first compared eDNA extraction methods and found that a soil extraction kit (Qiagen PowerSoil) was the most effective for detecting Batrachochytrium dendrobatidis in water samples. The PowerSoil extraction had a minimum detectio..View full abstract
Awarded by US National Science Foundation (IOS: Project)
Awarded by US Department of Defense (SERDP: Project)
This work was funded by the University of Pittsburgh's Dietrich School of Arts and Sciences, the US National Science Foundation (IOS: Project No. 1649443) and the US Department of Defense (SERDP: Project No. RC-2638).