Book Chapter

Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis.

Dhana G Gorasia, Eric Hanssen, Paul D Veith, Eric C Reynolds

Periodontal Pathogens, Methods and Protocols | Methods in Molecular Biology | SpringerLink | Published : 2021


The type IX secretion system (T9SS) is the most recently discovered secretion system in the gram-negative bacteria and is specific to the Bacteroidetes phylum. It is comprised of at least 19 proteins, which together allows for the secretion and cell surface attachment of a specific group of proteins (T9SS substrates), that harbor a signal sequence at the C-terminus. Here we describe the structural characterization of the PorK, PorN and PorG components of the Porphyromonas gingivalis T9SS using electron microscopy and cross-linking mass spectrometry.