Journal article

DynaMut2: Assessing changes in stability and flexibility upon single and multiple point missense mutations

Carlos HM Rodrigues, Douglas EV Pires, David B Ascher

Protein Science | WILEY | Published : 2020

Abstract

Predicting the effect of missense variations on protein stability and dynamics is important for understanding their role in diseases, and the link between protein structure and function. Approaches to estimate these changes have been proposed, but most only consider single-point missense variants and a static state of the protein, with those that incorporate dynamics are computationally expensive. Here we present DynaMut2, a web server that combines Normal Mode Analysis (NMA) methods to capture protein motion and our graph-based signatures to represent the wildtype environment to investigate the effects of single and multiple point mutations on protein stability and dynamics. DynaMut2 was ab..

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