Journal article

Monitoring DNA Damage and Repair in Peripheral Blood Mononuclear Cells of Lung Cancer Radiotherapy Patients

Pavel N Lobachevsky, Nicholas W Bucknell, Joel Mason, Diane Russo, Xiaoyu Yin, Lisa Selbie, David L Ball, Tomas Kron, Michael Hofman, Shankar Siva, Olga A Martin

Cancers | MDPI | Published : 2020

Abstract

Thoracic radiotherapy (RT) is required for the curative management of inoperable lung cancer, however, treatment delivery is limited by normal tissue toxicity. Prior studies suggest that using radiation-induced DNA damage response (DDR) in peripheral blood mononuclear cells (PBMC) has potential to predict RT-associated toxicities. We collected PBMC from 38 patients enrolled on a prospective clinical trial who received definitive fractionated RT for non-small cell lung cancer. DDR was measured by automated counting of nuclear γ-H2AX foci in immunofluorescence images. Analysis of samples collected before, during and after RT demonstrated the induction of DNA damage in PBMC collected shortly af..

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Grants

Awarded by CANCER AUSTRALIA Priority-drive Collaborative Cancer Research Scheme Grant


Funding Acknowledgements

This research was funded by CANCER AUSTRALIA Priority-drive Collaborative Cancer Research Scheme Grant 2013, grant number APP1060919