Journal article

Utility of ultra-sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities

Maria Gruenberg, Clara Antunes Moniz, Natalie E Hofmann, Cristian Koepfli, Leanne J Robinson, Elma Nate, Wuelton Marcelo Monteiro, Gisely Cardoso de Melo, Andrea Kuehn, Andre M Siqueira, Wang Nguitragool, Quique Bassat, Marcus Lacerda, Jetsumon Sattabongkot, Ivo Mueller, Ingrid Felger

Malaria Journal | BMC | Published : 2020

Abstract

BACKGROUND: The use of molecular diagnostics has revealed an unexpectedly large number of asymptomatic low-density malaria infections in many malaria endemic areas. This study compared the gains in parasite prevalence obtained by the use of ultra-sensitive (us)-qPCR as compared to standard qPCR in cross-sectional surveys conducted in Thailand, Brazil and Papua New Guinea (PNG). The compared assays differed in the copy number of qPCR targets in the parasite genome. METHODS: Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) parasites were quantified by qPCR amplifying the low-copy Pf_ and Pv_18S rRNA genes or the multi-copy targets Pf_varATS and Pv_mtCOX1. Cross-sectional surveys at the thr..

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Grants

Awarded by Swiss National Science Foundation


Awarded by TransEPi Consortium/Bill and Melinda Gates Foundation


Awarded by NIH


Funding Acknowledgements

This study was funded by the Swiss National Science Foundation (grants no. 310030_159580 and IZRJZ3_164182), the TransEPi Consortium/Bill and Melinda Gates Foundation (OPP1034577) and by the NIH (1 U19 AI089686-01). IM was supported by an NHMRC Senior Research Fellowship.