Journal article

Ternary Cu(II) Complex with GHK Peptide andCis-Urocanic Acid as a Potential Physiologically Functional Copper Chelate

Karolina Bossak-Ahmad, Marta D Wisniewska, Wojciech Bal, Simon C Drew, Tomasz Fraczyk

International Journal of Molecular Sciences | MDPI | Published : 2020


The tripeptide NH2-Gly-His-Lys-COOH (GHK), cis-urocanic acid (cis-UCA) and Cu(II) ions are physiological constituents of the human body and they co-occur (e.g., in the skin and the plasma). While GHK is known as Cu(II)-binding molecule, we found that urocanic acid also coordinates Cu(II) ions. Furthermore, both ligands create ternary Cu(II) complex being probably physiologically functional species. Regarding the natural concentrations of the studied molecules in some human tissues, together with the affinities reported here, we conclude that the ternary complex [GHK][Cu(II)][cis-urocanic acid] may be partly responsible for biological effects of GHK and urocanic acid described in the literatu..

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Awarded by National Science Center (Poland)

Funding Acknowledgements

This study was financed partly by National Science Center (Poland) Project 2016/23/B/ST5/02253. The equipment used was sponsored in part by the Centre for Preclinical Research and Technology (CePT), a project cosponsored by the European Regional Development Fund and Innovative Economy, The National Cohesion Strategy of Poland. S.C.D. was supported in part by a fellowship awarded from the Faculty of Medicine, Dentistry and Health Sciences, the University of Melbourne.