Human Milk Sampling Protocols Affect Estimation of Infant Lipid Intake
Alexandra D George, Melvin CL Gay, Kevin Murray, Beverly S Muhlhausler, Mary E Wlodek, Donna T Geddes
Journal of Nutrition | OXFORD UNIV PRESS | Published : 2020
BACKGROUND: Human milk (HM) lipid content is highly variable, and infants consume different volumes of milk. This makes precise sampling and calculation of the infant lipid intake problematic. OBJECTIVES: In order to describe inaccuracies of estimates of lipid content introduced by various sampling protocols, we compared the true infant lipid intake with estimated intakes using different milk sampling protocols. METHODS: Monthly milk samples (n = 1026) from months 1 to 6 of lactation were collected from 20 healthy, exclusively breastfeeding women. Infant lipid intake was measured by 24-hour test-weighing at month 3. Total lipid content was measured by creamatocrit. Concentrations and infant ..View full abstract
ADG is supported by a Medela AG Postgraduate Scholarship. BSM receives a Career Development Award from the National Health and Medical Research Council of Australia. MEW receives funding from the National Health and Medical Research Council of Australia. DTG is funded by an unrestricted research grant from Medela AG. MCLG and KM, no conflicts of interest.