Polymyxin-Induced Cell Death of Human Macrophage-Like THP-1 and Neutrophil-Like HL-60 Cells Associated with the Activation of Apoptotic Pathways
Ahmed M Fathalla, Seong H Chow, Thomas Naderer, Qi Tony Zhou, Tony Velkov, Mohammad AK Azad, Jian Li
Antimicrobial Agents and Chemotherapy | AMER SOC MICROBIOLOGY | Published : 2020
Innate immunity is crucial for the host to defend against infections, and understanding the effect of polymyxins on innate immunity is important for optimizing their clinical use. In this study, we investigated the potential toxicity of polymyxins on human macrophage-like THP-1 and neutrophil-like HL-60 cells. Differentiated THP-1 human macrophages (THP-1-dMs) and HL-60 human neutrophils (HL-60-dNs) were employed. Flow cytometry was used to measure the concentration-dependent effects (100 to 2,500 μM for THP-1-dMs and 5 to 2,500 μM for HL-60-dNs) and time-dependent effects (1,000 μM for THP-1-dMs and 300 μM for HL-60-dNs) of polymyxin B over 24 h. Effects of polymyxin B on mitochondrial acti..View full abstract
Awarded by National Institute of Allergy and Infectious Diseases of the National Institutes of Health
J.L., T.V., and T.Q.Z. are supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (grants R01 AI132681 and AI146160). A.M.F. is supported by a Monash Graduate Scholarship (MGS) and by a Monash International Postgraduate Research Scholarship (MIPRS).