Journal article

An Improved 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium Proliferation Assay to Overcome the Interference of Hydralazine

Yutang Wang, Dinh Tam Nguyen, Guang Yang, Jack Anesi, Zhonglin Chai, Fadi Charchar, Jonathan Golledge

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES | MARY ANN LIEBERT, INC | Published : 2020

Abstract

The MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay is one of the most commonly used assays to assess cell proliferation and cytotoxicity, but is subject to interference by testing compounds. Hydralazine, an antihypertensive drug, is commonly investigated in multiple fields such as heart failure, cancer, and blood pressure research. This study reported interference of the MTS assay by hydralazine and a simple modification overcoming this interference. Vascular smooth muscle cells were cultured in the presence or absence of hydralazine (0, 10, 50,100, and 500 μM) for 2 or 24 h. Cell numbers were analyzed using MTS, trypan blue exclusion, or..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by National Health and Medical Research Council of Australia (NHMRC)


Funding Acknowledgements

This work was funded by grants from the National Health and Medical Research Council of Australia (1062671). J.G. holds a Practitioner Fellowship from the National Health and Medical Research Council of Australia (NHMRC; 1117061) and a Senior Clinical Research Fellowship from the Queensland Government, Australia.