Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell line

Melanie K Stewart; Deidre M Mattiske; Andrew J Pask

Journal article

Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell line

Melanie K Stewart, Deidre M Mattiske, Andrew J Pask

BMC MOLECULAR AND CELL BIOLOGY | BMC | Published : 2020

DOI: 10.1186/s12860-020-00307-9

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Abstract

Background The increasing incidence of reproductive disorders in humans has been attributed to in utero exposure to estrogenic endocrine disruptors. In particular, exposure of the developing testis to exogenous estrogen can negatively impact male reproductive health. To determine how estrogens impact human gonad function, we treated the human testis-derived cell line NT2/D1 with estrogen and examined its impact on SOX9 and the expression of key markers of granulosa (ovarian) and Sertoli (testicular) cell development. Results Estrogen successfully activated its cognate receptor (estrogen receptor alpha; ESR1) in NT2/D1 cells. We observed a significant increase in cytoplasmic SOX9 following e..

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University of Melbourne Researchers

Deidre Mattiske Author

Andrew Pask Author

Related Projects (1)

ENDOCRINE CONTROL OF CELL FATE IN THE GONAD

Disorders of sexual development are amongst the most common birth defects in humans. These conditions have profound consequences for the phy..

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Awarded by NHMRC

Funding Acknowledgements

This work was supported by an NHMRC Project Grant APP1142481 to AJP. The funding body played no role in the design of the study and collection, analysis, interpretation of data or in writing the manuscript.