Journal article

UTX coordinates steroid hormone-mediated autophagy and cell death

Donna Denton, May T Aung-Htut, Nirmal Lorensuhewa, Shannon Nicolson, Wenying Zhu, Kathryn Mills, Dimitrios Cakouros, Andreas Bergmann, Sharad Kumar

NATURE COMMUNICATIONS | NATURE PUBLISHING GROUP | Published : 2013

Abstract

Correct spatial and temporal induction of numerous cell type-specific genes during development requires regulated removal of the repressive histone H3 lysine 27 trimethylation (H3K27me3) modification. Here we show that the H3K27me3 demethylase dUTX is required for hormone-mediated transcriptional regulation of apoptosis and autophagy genes during ecdysone-regulated programmed cell death of Drosophila salivary glands. We demonstrate that dUTX binds to the nuclear hormone receptor complex Ecdysone Receptor/Ultraspiracle, and is recruited to the promoters of key apoptosis and autophagy genes. Salivary gland cell death is delayed in dUTX mutants, with reduced caspase activity and autophagy that ..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) Project


Awarded by Senior Principal Research Fellowship


Awarded by National Institutes of Health (NIH)


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

This work was supported by the National Health and Medical Research Council (NHMRC) Project Grants 626903 and 626923, and a Senior Principal Research Fellowship 1002863 to S. K., as well as R01 GM068016 from the National Institutes of Health (NIH) to A. B. We thank the Australian Drosophila Research Support Facility, Vienna Drosophila RNAi Center and Bloomington Drosophila Stock Center for Drosophila stocks, Developmental Studies Hybridoma Bank (University of Iowa) for antibodies,