A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling
Sonia Sanz Munoz, Martin Engel, Rachelle Balez, Dzung Do-Ha, Mauricio Castro Cabral-da-Silva, Damian Hernandez, Tracey Berg, Jennifer A Fifita, Natalie Grima, Shu Yang, Ian P Blair, Garth Nicholson, Anthony L Cook, Alex W Hewitt, Alice Pebay, Lezanne Ooi
Cells | MDPI | Published : 2020
The study of neurodegenerative diseases using pluripotent stem cells requires new methods to assess neurodevelopment and neurodegeneration of specific neuronal subtypes. The cholinergic system, characterized by its use of the neurotransmitter acetylcholine, is one of the first to degenerate in Alzheimer's disease and is also affected in frontotemporal dementia. We developed a differentiation protocol to generate basal forebrain-like cholinergic neurons (BFCNs) from induced pluripotent stem cells (iPSCs) aided by the use of small molecule inhibitors and growth factors. Ten iPSC lines were successfully differentiated into BFCNs using this protocol. The neuronal cultures were characterised thro..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
Awarded by NHMRC Boosting Dementia Research Leadership Fellowship
Awarded by NHMRC Senior Research Fellowship
The research was funded by National Health and Medical Research Council (NHMRC) of Australia grants (APP1071250, and APP1095215) and a NHMRC Boosting Dementia Research Leadership Fellowship awarded to LO (APP1135720). AP is supported by a NHMRC Senior Research Fellowship (1154389).