Evaluation of Bluetongue Virus (BTV) Antibodies for the Immunohistochemical Detection of BTV and Other Orbiviruses
Fabian ZX Lean, Jean Payne, Jennifer Harper, Joanne Devlin, David T Williams, John Bingham
Microorganisms | MDPI | Published : 2020
The detection of bluetongue virus (BTV) antigens in formalin-fixed tissues has been challenging; therefore, only a limited number of studies on suitable immunohistochemical approaches have been reported. This study details the successful application of antibodies for the immunohistochemical detection of BTV in BSR variant baby hamster kidney cells (BHK-BSR) and infected sheep lungs that were formalin-fixed and paraffin-embedded (FFPE). BTV reactive antibodies raised against non-structural (NS) proteins 1, 2, and 3/3a and viral structural protein 7 (VP7) were first evaluated on FFPE BTV-infected cell pellets for their ability to detect BTV serotype 1 (BTV-1). Antibodies that were successful i..View full abstract
This work is supported by the CSIRO Australian Centre for Diseases Preparedness (ACDP, formerly AAHL). Fabian Lean was supported by a Research Training Grant of the Australian Commonwealth and the University of Melbourne.