An Assessment of the Molecular Diversity of Ticks and Tick-Borne Microorganisms of Small Ruminants in Pakistan
Abdul Ghafar, Adil Khan, Alejandro Cabezas-Cruz, Charles G Gauci, Sadaf Niaz, Sultan Ayaz, Lourdes Mateos-Hernandez, Clemence Galon, Nasreen Nasreen, Sara Moutailler, Robin B Gasser, Abdul Jabbar
Microorganisms | MDPI AG | Published : 2020
This study investigated ticks and tick-borne microorganisms of small ruminants from five districts of the Federally Administered Tribal Area (FATA) of Pakistan. Morphological (n = 104) and molecular (n = 54) characterization of the ticks revealed the presence of six ixodid ticks: Rhipicephalus (Rh.) haemaphysaloides, Rh. microplus, Rh. turanicus, Haemaphysalis (Hs.) punctata, Hs. sulcata and Hyalomma anatolicum. Phylogenetic analyses of nucleotide sequence data for two mitochondrial (16S and cytochrome c oxidase 1) and one nuclear (second internal transcribed spacer) DNA regions provided strong support for the grouping of the six tick species identified in this study. Microfluidic real-time ..View full abstract
This research received no specific grant from any funding agency, commercial or not-for-profit sectors. The visit of A.K. to the University of Melbourne was funded by the Higher Education Commission of Pakistan.