Book Chapter

The IVF Cycle to Come: Laboratory Innovations

Denny Sakkas, David K Gardner

Patient-Centered Assisted Reproduction. How to Integrate Exceptional Care with Cutting-Edge Technology | Cambridge University Press | Published : 2020

Abstract

In the first reported case of IVF and embryo development in the human, Rock and Menkin made the following description of the culture and development of a fertilized embryo: “The eggs were incubated in serum for 22.5 hours, being washed in salt solution before and after incubation, and then exposed to a washed sperm suspension in Locke’s solution for two hours at room temperature. They were again washed in Locke’s solution and cultured in fresh serum for 45 hours.

University of Melbourne Researchers