Journal article

Cancer-associated fibroblasts of the prostate promote a compliant and more invasive phenotype in benign prostate epithelial cells

A Jaeschke, A Jacobi, MG Lawrence, GP Risbridger, M Frydenberg, ED Williams, I Vela, DW Hutmacher, LJ Bray, A Taubenberger

Materials Today Bio | ELSEVIER | Published : 2020

Abstract

Reciprocal interactions between prostate epithelial cells and their adjacent stromal microenvironment not only are essential for tissue homeostasis but also play a key role in tumor development and progression. Malignant transformation is associated with the formation of a reactive stroma where cancer-associated fibroblasts (CAFs) induce matrix remodeling and thereby provide atypical biochemical and biomechanical signals to epithelial cells. Previous work has been focused on the cellular and molecular phenotype as well as on matrix stiffness and remodeling, providing potential targets for cancer therapeutics. So far, biomechanical changes in CAFs and adjacent epithelial cells of the prostate..

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Grants

Awarded by Victorian Government through the Victorian Cancer Agency


Awarded by National Health and Medical Research Council


Funding Acknowledgements

This project was supported by an Australia-Germany Joint Research Co-operation grant with the German Academic Exchange Service (DAAD) awarded to LJB. AJ was supported by a Postgraduate Research Award (International), QUT. MGL was supported by the Victorian Government through the Victorian Cancer Agency (Fellowship MCRF18017). GPR was supported by the National Health and Medical Research Council (Fellowship 1102752). We would like to thank the patients who generously donated their tissue for our research. We also thank Melissa Papargiris, TissuPath, the Australian Prostate Cancer BioResource and the Melbourne Urological Research Alliance for obtaining the prostate tissues. We gratefully thank Prof. Jochen Guck and his team for great support and scientific discussions. We acknowledge the CMCB light microscopy facility (in part funded by the state of Saxony and the European Fund for Regional Development-EFRE) and Monash Histology Platform for support. We thank JPK/Bruker instruments for technical support. Some of the data reported in this work were obtained at the Central Analytical Research Facility (CARF) operated by the Institute for Future Environments, QUT. Access to CARF is supported by the Science and Engineering Faculty, QUT. The authors acknowledge the TRI for providing the excellent research environment and core facilities that enabled this research. We particularly thank Dorothy Loo-Oey and the team from the Proteomics Core Facility for their continuous support.