DNA Methylation in Peripheral Blood and Risk of Gastric Cancer: A Prospective Nested Case-control Study
James A Chamberlain, Pierre-Antoine Dugue, Julie K Bassett, Roger L Milne, Jihoon E Joo, Ee Ming Wong, Maree T Brinkman, Geoffrey W Stuart, Alex Boussioutas, Melissa C Southey, Graham G Giles, Hazel Mitchell, Dallas R English, Allison M Hodge
CANCER PREVENTION RESEARCH | AMER ASSOC CANCER RESEARCH | Published : 2021
DNA methylation in peripheral blood is a potential biomarker of gastric cancer risk which could be used for early detection. We conducted a prospective case-control study nested within the Melbourne Collaborative Cohort Study. Genomic DNA was prepared from blood samples collected a median of 12 years before diagnosis for cases (N = 168). Controls (N = 163) were matched to cases on sex, year of birth, country of birth, and blood sample type using incidence density sampling. Genome-wide DNA methylation was measured using the Infinium HumanMethylation450K Beadchip. Global measures of DNA methylation were defined as the median methylation M value, calculated for each of 13 CpG subsets representi..View full abstract
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Awarded by Australian National Health and Medical Research Council
The MCCS cohort recruitment was funded by VicHealth and Cancer Council Victoria. The MCCSwas further augmented by Australian National Health and Medical Research Council grants 209057, 396414, 1074383, and 1087683 and by infrastructure provided by Cancer Council Victoria. The funding sources had no role in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication.