Shedding light on isomeric FAHFA lipid structures using 213 nm ultraviolet photodissociation mass spectrometry
Edgar White Buenger, Gavin E Reid
EUROPEAN JOURNAL OF MASS SPECTROMETRY | SAGE PUBLICATIONS LTD | Published : 2020
Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) are a recently discovered class of biological active lipids with anti-diabetic and anti-inflammatory functions. Given that structure and function are intimately related, we report here the use of direct infusion multi-stage hybrid tandem mass spectrometry involving sequential Collisional Activated Dissociation (CAD) and 213 nm UltraViolet PhotoDissociation (UVPD), as a novel technique for the unambiguous denovo identification and detailed structural characterisation of FAHFA lipid ions, including determination of the esterified fatty acid identity, the hydroxy fatty acid identity and position of esterification, and localization of the site(s)..View full abstract
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Awarded by Australian Research Council
The author(s) disclosed the receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported by funding from the Australian Research Council (DP190102464) to GER.