Journal article

Identification and characterization of the long noncoding RNA Dreg1 as a novel regulator of Gata3

Wing Fuk Chan, Hannah D Coughlan, Nadia Iannarella, Gordon K Smyth, Timothy M Johanson, Christine R Keenan, Rhys S Allan

Immunology & Cell Biology | WILEY | Published : 2020

Abstract

The eukaryotic genome is three-dimensionally segregated into discrete globules of topologically associating domains (TADs), within which numerous cis-regulatory elements such as enhancers and promoters interact to regulate gene expression. In this study, we identify a T-cell-specific sub-TAD containing the Gata3 locus, and reveal a previously uncharacterized long noncoding RNA (Dreg1) within a distant enhancer lying approximately 280 kb downstream of Gata3. Dreg1 expression is highly correlated with that of Gata3 during early immune system development and T helper type 2 cell differentiation. Inhibition and overexpression of Dreg1 suggest that it may be involved in the establishment, but not..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Australian Research Council


Funding Acknowledgements

We thank the staff of the core facilities at the Walter and Eliza Hall Institute, particularly the Flow Cytometry facility (WEHIFACS) and the Bioservices department. This work was supported by grants and fellowships from the National Health and Medical Research Council of Australia (CRK #1125436, TMJ #1124081, RSA #1100451, GKS & RSA #1158531), the Australian Research Council (RSA # 130100541), and the Flack Trust (HDC Marian and E.H. Flack Fellowship). This study was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC Independent Research Institute Infrastructure Support scheme and the Australian Cancer Research Fund.