Journal article
Identification of the skeletal progenitor cells forming osteophytes in osteoarthritis
AJ Roelofs, K Kania, AJ Rafipay, M Sambale, ST Kuwahara, FL Collins, J Smeeton, MA Serowoky, L Rowley, H Wang, R Gronewold, C Kapeni, S Méndez-Ferrer, CB Little, JF Bateman, T Pap, FV Mariani, J Sherwood, JG Crump, C De Bari
Annals of the Rheumatic Diseases | BMJ PUBLISHING GROUP | Published : 2020
Abstract
Objectives Osteophytes are highly prevalent in osteoarthritis (OA) and are associated with pain and functional disability. These pathological outgrowths of cartilage and bone typically form at the junction of articular cartilage, periosteum and synovium. The aim of this study was to identify the cells forming osteophytes in OA. Methods Fluorescent genetic cell-labelling and tracing mouse models were induced with tamoxifen to switch on reporter expression, as appropriate, followed by surgery to induce destabilisation of the medial meniscus. Contributions of fluorescently labelled cells to osteophytes after 2 or 8 weeks, and their molecular identity, were analysed by histology, immunofluoresce..
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Awarded by Murdoch Children's Research Institute
Funding Acknowledgements
CDB, AJRo, KK, AJRa, FLC and HW were supported by funding from Versus Arthritis, formerly Arthritis Research UK (20775, 21156, 20050, 19429), and the Medical Research Council (MR/L020211/1). TP, JSh, MSa and RG were supported by funding from the Bundesministerium fur Bildung und Forschung (BMBF) Overload-PrevOP consortium (01EC1408F) and the Innovative Medizinische Forschung (IMF) Programme of the University Hospital Munster (Project I--SH121608). JGC, STK, JSm, MAS and FVM were supported by funding from the National Insitute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS: R01AR069700). JFB, LR and CBL were supported by the National Health and Medical Research Council (NHMRC: APP1063133), and part funding was provided to JFB by the Victorian Government's Operational Infrastructure Support Programme to the Murdoch Children's Research Institute. CK and SMF were supported by funding from the Wellcome Trust (203151/Z/16/Z), Horizon2020 (ERC-2014--CoG-648765), Cancer Research UK (C61367/A26670) and NHS Blood and Transplant.