Sodium nitrate co-supplementation does not exacerbate low dose metronomic doxorubicin-induced cachexia in healthy mice
Dean G Campelj, Danielle A Debruin, Cara A Timpani, Alan Hayes, Craig A Goodman, Emma Rybalka
SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2020
The purpose of this study was to determine whether (1) sodium nitrate (SN) treatment progressed or alleviated doxorubicin (DOX)-induced cachexia and muscle wasting; and (2) if a more-clinically relevant low-dose metronomic (LDM) DOX treatment regimen compared to the high dosage bolus commonly used in animal research, was sufficient to induce cachexia in mice. Six-week old male Balb/C mice (n = 16) were treated with three intraperitoneal injections of either vehicle (0.9% NaCl; VEH) or DOX (4 mg/kg) over one week. To test the hypothesis that sodium nitrate treatment could protect against DOX-induced symptomology, a group of mice (n = 8) were treated with 1 mM NaNO3 in drinking water during DO..View full abstract
This study was funded by former Institute of Sport, Exercise and Active Living, and Centre for Chronic Disease, program grants (both Victoria University).