Evaluation of Dental Pulp Stem Cell Heterogeneity and Behaviour in 3D Type I Collagen Gels
Amr Alraies, Rachel J Waddington, Alastair J Sloan, Ryan Moseley
BIOMED RESEARCH INTERNATIONAL | HINDAWI LTD | Published : 2020
Dental pulp stem cells (DPSCs) are increasingly being advocated for regenerative medicine-based therapies. However, significant heterogeneity in the genotypic/phenotypic properties of DPSC subpopulations exist, influencing their therapeutic potentials. As most studies have established DPSC heterogeneity using 2D culture approaches, we investigated whether heterogeneous DPSC proliferative and contraction/remodelling capabilities were further evident within 3D type I collagen gels in vitro. DPSC subpopulations were isolated from human third molars and identified as high/low proliferative and multipotent/unipotent, following in vitro culture expansion and population doubling (PD) analysis. High..View full abstract
This work was supported by a PhD studentship awarded to Dr. Amr Alraies by Albawani Company, Saudi Arabia. The authors also wish to thank Dr. Nafiseh Badiei (College of Engineering, Swansea University, UK), for her help in undertaking the Scanning Electron Microscopy (SEM) analysis of the acellular and DPSC-seeded type I collagen gels.