Journal article

Analyzing the shear-induced sensitization of mechanosensitive ion channel Piezo-1 in human aortic endothelial cells

Austin Lai, Yung C Chen, Charles D Cox, Anthony Jaworowski, Karlheinz Peter, Sara Baratchi

Journal of Cellular Physiology | WILEY | Published : 2020

Abstract

Mechanosensitive ion channels mediate endothelial responses to blood flow and orchestrate their physiological function in response to hemodynamic forces. In this study, we utilized microfluidic technologies to study the shear-induced sensitization of endothelial Piezo-1 to its selective agonist, Yoda-1. We demonstrated that shear stress-induced sensitization is brief and can be impaired when exposing aortic endothelial cells to low and proatherogenic levels of shear stress. Our results suggest that shear stress-induced sensitization of Piezo-1 to Yoda-1 is independent of cell-cell adhesion and is mediated by the PI3K-AKT signaling pathway. We also found that shear stress increases the membra..

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Grants

Awarded by Australian Research Council


Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

Australian Research Council, Grant/Award Numbers: DE170100239, DP200101248; Australian National Health and Medical Research Council, Grant/Award Numbers: APP1079492, APP1128046